The aims were as follows: (1) to evaluate the effects at 48 and 96 h of two concentrations (25 and 50%) of leukocyte and platelet-rich\ngel (L-PRG) and pure PRG (P-PRG) supernatants on the production/degradation in normal equine synovial membrane explants\n(SME) of platelet derived growth factor isoform BB, transforming growth factor beta-1, tumor necrosis factor alpha, interleukin\n(IL-) 4 (IL-4), IL-1 receptor antagonist (IL-1ra), and hyaluronan (HA) synthesis and (2) to correlate these molecules with their\nrespective PRG supernatant treatments. SME from 6 horses were cultured for 96h with L-PRG and P-PRG supernatants at 25\nand 50% concentrations, respectively. SME culture media were changed each 48 h and used for determination by ELISA of the\nmolecules, which were also determined in synovial fluid. 25% L-PRG supernatant produced a sustained release over time of IL-1ra\nand a gradual release of HA, whereas 50% L-PRG supernatant produced a sustained increase over time of IL-4 and HA. 50% PPRG\nsupernatant produced an increased and sustained production of IL-1ra and IL-4. The cellular composition and the articular\nconcentration (volume) of a platelet-rich plasma preparation could affect the anti-inflammatory and anabolic joint responses in\nhorses with osteoarthritis.
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